FISH is a powerful tool for determining many types of chromosome anomalies. In addition to the AneuVysion Assay, the only FDA cleared product for prenatal aneuploidy detection, Vysis offers an expansive line of DNA FISH probes for evaluation of chromosome anomalies in preimplantation, prenatal and postnatal genetic testing and research.

The advantages of AneuVysion for prenatal testing include:

Rapid information on chromosome abnormalities, especially critical in medically urgent situations
Aneuploidy analysis on specimens that do not provide metaphase cells for analysis - such as in situations of culture failure
DNA FISH Probes for microdeletion syndrome

Vysis microdeletion probes provide unique advantages for detection of deleted genes and loci associated with microdeletion syndromes. The microdeletion probes consist of a probe specific for the locus or region of deletion associated with the microdeletion syndrome. Also included is a control probe for accurate identification of the chromosome of interest.

Vysis' microdeletion probes provide same day FISH results for minimal hands-on time, saving laboratory expenses and resources.

DNA FISH Probes for Oncology and Pathology

Genetic anomalies associated with cancer continue to be discovered through the use of FISH, CGH and other molecular technologies. The Vysis PathVysion™ HER-2 DNA Probe Kit has been approved by the FDA to assess HER-2 status in breast cancer. In addition to the CEP Chromosome Enumeration Kits, CEP8, CEP12 and CEP XY, the only FDA-cleared FISH products for assessment of myeloid disorders and sex-mismatched bone marrow transplantation, Vysis offers an extensive line of clinical and research DNA FISH probes for identification of genetic and chromosomal aberrations. Solid tumor probes have been optimized for paraffin embedded tissues.

WCP Chromosome Paint and SpectraVysion DNA FISH Probes

The combination of new, high quality amplified chromosomal DNA methods and Vysis' patented fluorophore direct labeling technology results in second generation WCP DNA FISH probes that produce brighter fluorescent signals. Manufactured to meet new design specifications for intense fluorescence through a triple bandpass filter set (DAPI/ Green/Orange), improved chromosome coverage, and specificity, the WCP probes are designed to better meet your research requirements for:

Chromosome translocation detection
Multiple chromosome rearrangement analysis
Chromosome rearrangement studies or mutagenesis, radiation or chemical dosimetry
Marker chromosome detection
Human chromosome detection in animal/human cell hybrids
CEP Chromosome Enumeration DNA FISH Probes

CEP probes consist of chromosome-specific sequences from highly repeated human satellite DNA sequences, usually located at the centromere region of the chromosome. CEP probes enable researchers to identify and enumerate human chromosomes in interphase and metaphase cells. Through a fluorescence microscope, probe signals appear as compact fluorescent "spots" where the CEP probe is hybridized to the target DNA in the chromosome.

Users can determine chromosome-specific ploidy on solid tumors, fresh or archived biopsy specimens, and on cultured and uncultured preimplantation, prenatal, postnatal, and hematologic samples. Chromosome-specific ploidy analysis is increasingly becoming a critical technique for clinical researchers in areas like breast and myeloid cancers.

LSI Unique Sequence DNA FISH Probes

LSI probes consist of DNA probe sequences homologous to specific human genes or loci and are premixed with blocking DNA to exclude hybridization to other loci. LSI probes hybridize to specific locations on indicated chromosomes and are gene, locus or region specific. The LSI probes quickly identify whether specific genes, loci, or regions, are present or if deletions or amplifications have occurred. This ability provides outstanding potential for use in cancer, prenatal, postnatal, preimplantation and other genetic disease research.

LSI probes are designed to evaluate specific human genes, loci, or regions on chromosomes in either metaphase or interphase cells, allowing researchers to conduct studies directed at establishing their clinical relevance.

TelVision DNA FISH Probes for Telomeric regions

Telomeric regions lost due to deletions and unbalanced translocations have been associated with idiopathic mental retardation and polycystic kidney disease. Research has shown that subtelomeric DNA contains a high density of genes. DNA probes for the terminal regions of chromosome arms are proving to be useful research tools for a number of disorders involving the telomeric regions.

Vysis TelVysion probes are designed to detect the chromosome specific subtelomeric regions of the human chromosomes. The TelVysion probes provide the following advantages for the research laboratory using FISH to detect cryptic translocations in specimens obtained for prenatal, congenital and cancer research.

DNA Fluorescent Labelling reagents for FISH

The Vysis fluorophore-labeled dUTP products and the Nick Translation Kit are designed for the researcher interested in labeling DNA probes for FISH. Vysis' DNA labeling products provide excellent performance and include protocols that simplify fluorescent DNA probe labeling.

In Situ Hybridization reagents & accessories

Vysis in situ hybridization reagents are essential for a quality FISH result. Vysis probes were developed to be specifically matched with Vysis hybridization reagents for optimum results. Vysis now offers all the necessary accessory reagents and equipment to perform FISH. This provides a great convenience to the clinical or research laboratory through single-source.