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Edinburgh Instruments Analytical Division is a world leader in the design and manufacture of single photon counting sensitive steady state and time resolved fluorescence and phosphorescence lifetime spectrometers.
Based on single photon counting techniques, the products are designed to meet the highest level of technical performance and reliability. The modular construction of the spectrometers enables systems to be flexibly configured to meet the individual needs of the researcher.
The company also offers a turn-key Laser Flash Photolysis spectrometer. This spectrometer has been designed to measure laser induced transient absorption and emission kinetics and the associated transient spectra. The spectrometer can be supplied with PMT based detection or near IR detector for transient studies or with an array detector for simultaneous multiple wavelength acquisition.
The Laboratory Teaching Kit, in which you have shown interest, uses the mini-τ spectrometer – the world’s smallest fluorescence lifetime spectrometer based on time correlated single photon counting. This teaching kit is designed to instruct students in the measurement technique and analysis of fluorescence lifetime kinetics using the example of one of the most used fuorophores – Fluorescein. This kit will introduce time correlated single photon counting (TCSPC) as the method of choice for fluorescence lifetime measurements.
The kit comprises a mini-τ spectrometer with picosecond pulse laser excitation, a dedicated computer, acquisition and analysis software, and a set of readily prepared samples.
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The parameters affecting fluorcence emission are
- polarity
- pH
- pressure
- hydrogen bonds
- temperature
- viscosity
- ion and
- quenching
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What does the fluorescein lifetime teaching kit consist of?
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| mini – τ |
Single photon counting sensitive fluorescence lifetime spectrometer based on Time Correlated Single Photon Counting, including personal computer with plug-in data acquisition card;
Single photon counting photomultiplier, sensitive to 650 nm;
Single sample holder with cooling option and temperature probe
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| Excitation source |
Picosecond diode laser EPL 475: pulse width < 90 ps, λ= (475 ± 10) nm |
| Software |
T900 software for data acquisition and decay analysis of up to 4 exponentials |
| Dye and reagents |
Fluorescein, titration solutions, labelled peptides, caspase-3 |
| Cuvettes |
1 pack of disposable fluorescence cuvettes |
| Technical literature |
Introduction to Fluorescence Lifetime measurements Introduction to Time Correlated Single Photon Counting mini-τ manual Theory of Fluorescence Lifetime Analysis Experiment work book |
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Skills developed when using Laboratory Teaching Kit from Edinburgh Instruments:
- "Working with lifetimes"
- Operating fluorescence lifetime
- instrumentation
- Performing lifetime measurements
- Insight into the time correlated single
- photon counting technique
- Analysing lifetime data
- Making use of the "Lifetime
- Advantage"
- Distinguishing between static and
- dynamic quenching
- Performing time resolved FRET
- measurements
- Analysing time resolved FRET
- experiments
- Using Time Resolved FRET for
- cleavage experiments
- Targeting complex biological tasks
- with fundamental knowledge of the
- fluorescence lifetime technology
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